The novel Q-VD-OPH compound synthesized using proprietary technology is an irreversible caspase inhibitor specifically designed for in-vivo research.The Q-VD-OPH irreversibily binds to activated Caspases to block apoptosis. Apoptosis is a physiological form of cell death, which plays an important role in embryogenesis, cellular homoestasis, tissue atrophy , and removal damaged and mutated cells. Caspases, which act as molecular chainsaws, are the central components of apoptosis response. Caspases are Cysteine proteases that cleave after aspartate residue in their substrates. At least 7 of 14 known mammalian caspases have important role in apoptosis The "OPH" trap with its superior potency, proven cell permeability and minimal toxicity provides an exceptional alternative to the Fluoromethylketone family of inhibitors. These two types of compounds may form the basis for important new drug candidates for a wide variety of disease targets.
Q-VD-OPh is a cell-permeable, irreversible inhibitor of caspase activity (Philipp J. Jost,2009). The "OPH" trap provides these important features: Nontoxic (preliminary study showed no evidence of toxicity in mice injected intraperitoneally with more than 1000 mg/kg) and Potency (Our new caspase inhibitors show significant improvement in potency. IC50 values against purified caspases are now in the nanomolar range. In an in vitro cell based assay, greater than 90% inhibition of apoptosis was achieved). Note: Optimal dilutions should be determined by the laboratory for each specific applications.
Purity: HPLC > 95%
Key Applications: Inhibitor, Caspase Inhibitor / Apoptosis Inhibitor
Application Areas: Cell Viability Analysis, Activators, Inhibitors & Substrates
Research Areas: Caspase Inhibitor
Product Type: Proteins, Enzymes & Peptides
Biochemical Category: Proteins, Enzymes & Peptides
Presentation: Off-white semi solid
Format: Off white semi solid
NOTES: Mass Spectrometry: M+1=514.0 ; M+Na = 536.2 ; Chromatography TLC Rf: 0.4 one spot, EtOAC: 6,Hex:4, ACOH:0.1 ; SOLUBILITY: DMSO ; Stability: > 1 year stored in desiccator, > 3 year stored at - 20°C
Solubility: For each mg of Q-VD-OPH: to make 20mM, add 97 µL DMSO to make 10mM, add 194 µL DMSO to make 5mM, add 388 µL DMSO, etc.
Storage & Handling: Keep Cool and Dry. Store reagent desiccated at 2 - 8 ° C upon arrival. Lyophilized, samples are stable at least for 1 year, desiccated, at 2 - 8 ° C. Upon reconstitution in 100% DMSO, stock concentrations of 10 - 20 mM are stable for 6 months at ≤ -20°C. Avoid repeated freeze/thaw cycles by aliquoting the stock solution prior to freezing.