Puromycin dihydrochloride, Ready Made Solution, from Streptomyces alboniger, 10 mg/mL in H2O, 0.2 mum filtered

CAS Number: 58-58-2

MDL No.: MFCD00012691

Purity: >98% (HPLC)

Storage: -20C

UNSPSC Code: 51101500

General description: Chemical structure: peptidyl nucleoside

Application: Puromycin is an aminonucleoside antibiotic that is derived from Streptomyces alboniger. It allows selection for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). It has been used to study vascular smooth muscle cell viability after treatment in the rabbit model . Puromycin is used to produce enhanced green fluorescent protein (EGFP) transgenic piglets after somatic cell cloning and embryo transfer.

Biochem/physiol Actions: Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination acting as an analog of the 3'-terminal end of the aminoacyl-tRNA. Puromycin also acts as a reversible inhibitor of dipeptidyl-peptidase II (serine peptidase) and cytosol alanyl aminopeptidase.Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene. Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days.

Preparation Note: This product is concentrated at 10 mg/mL in water and is 0.2 mum filtered. It has been tested on HeLa cells for cell growth arrest and selection of cells after transfection of the pac resistance gene.

Other Notes: Keep container tightly closed in a dry and well-ventilated place.