The total protein is determined using the Biuret procedure, with bovine albumin as the standard. Each vial is overfilled by approximately 10 percent so that a minimum of 50 mg of rabbit IgG is obtained upon reconstitution.
Rabbit IgG is purified from pooled rabbit serum using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption. The product is dialyzed into 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative added.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.
Purified rabbit IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Rabbit IgG was used to reduce non-specific binding in immunoprecipitation of protein extracts and as control antibody in radioimmunoassay, immunocytochemistry and immunohistochemistry. Rabbit IgG was also used as a standard to demonstrate the performance of land-contrast (LC) BioCD (protein array) and in neutralization assays. It is suitable for use as a positive control in antibody identification screening techniques.
Product is the lyophilized powder of rabbit IgG and buffer salts.
Reconstitution: Reconstitute product with 3 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Purity: > 99%, lyophilized
Assay: The product is tested for purity at a minimum of 20 mg/mL using immunoelectrophoresis. The product is rabbit IgG; no trace of IgA, IgM or non-immunoglobulin serum proteins is detected.
Concentration: Total Protein: 18.2 mg/mL, Volume: 3 mL
Key Applications: ELISA, Dot immunobinding, Western immunoblotting, Immunodiffusion, Immunoelectrophoresis
Application Areas: Immunoassays
Product Type: Reagents
Conjugate: Unconjugated
Isotype: IgG
Format: Lyophilized
NOTES: Purified protein
Storage & Handling: Store unopened product at 2-8°C. Store reconstituted material no longer than a few days at 2-8°C unless sodium azide is added. For long-term storage, aliquot and freeze at -20°C.