Protocatechuate 3,4-Dioxygenase from Pseudomonas sp., lyophilized powder, >=3 units/mg solid

CAS Number: 9029-47-4

MDL No.: MFCD00132132

Storage: -20C

Enzyme Commission (EC) Number: 1.13.11.3  ( BRENDA | IUBMB )

UNSPSC Code: 12352204

Application: The enzyme has been used to create an oxygen scavenging system along with protocatechuate (PCA) and Trolox. The enzyme employs a nonheme iron center that catalyzes the conversion of PCA and molecular oxygen into β-carboxy-cis,cis-muconic acid, while the antioxidant Trolox suppresses slow blinking and photobleaching of cyanine dyes. It has been used in the preparation of imaging buffer along with DMB-BSA (dynein motility buffer-BSA), ATP and protocatechuate in single molecule motility assay.

Application: Protocatechuate 3,4-Dioxygenase(PCD), from Pseudomonas sp., is used for the enzymatic determination of choline esterase when coupled with phydroxybenzoate hydroxylase. It is used to improve organic fluorophore-stability in single-molecule experiments and is used to study the metabolism of protocatechuate in Rhizobiaceae.

Physical form: Supplied as lyophilized powder containing approx. 40% stabilizer.

Physical properties: Structure : Protein with nonheme ironInhibitors : Ag+, Hg++, PCMBOptimum pH : 9.0 Optimum temperature : 60-65C pH Stability : pH 7.0-9.0 (25C, 72hr) Thermal stability : below 50C (pH 6.0, 1hr)

Unit Definition: One unit will oxidize 1.0 mumole of protocatechuate to 3-carboxy-cis,cis-muconate per min at pH 7.5 at 37 C.

Analysis Note: Protein determined by biuret.