Protein A affinity chromatography is widely used for antibody purification. However, the leakage of Protein A, though covalently conjugated to chromatography supports, posts a big problem for antibody based pharmaceutical industry where false results could be generated in immunological assays from contaminated Protein A.
GenScript Protein A ELISA Kit is developed for accurate quantification of the leakage of native Protein A, recombinant Protein A variants and MabSelect SuRe ™ in the presence or absence of antibodies. The kit offers pharmaceutical users a sensitive assay to measure mini amount of Protein A in therapeutic antibody products. It also facilitates Protein A resin manufacturers in monitoring leaching characteristics of their resins under specific conditions.
Key Features:
- Accurate detection of 20 pg/ml Protein A and 50 pg/ml MabSelect SuRe ™ in antibody preps
- Broad-range recongnition of native Protein A, recombinant Protein A variants and MabSelect SuRe ™
- Low level deviation - Intra-assay <5%, Inter-assay <10%
- High accuracy/recovery within 85-115%
- Ready-to-use ELISA plate with fast & simple samples preparation
- Time-saving protocol - complete assay in less than 2 hours
- Exceptional lot-to-lot consistency ensured by high-quality monoclonal antibodies
- Wide applications for the quantification of Protein A contaminant and leaching
Storage:
The kit is stable for 12 months if stored at 2-8 ?.
Do NOT freeze any component of the kit.