MDL number: MFCD00677817
Components
AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon
Application
E. coli cells
baculovirus infected
Spodoptera frugiperda pupal
ovary cells
Other Notes
Mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic, and aminopeptidases, and thermolysin-like activities. Formulated with no metal chelators that might inhibit binding of histidine-tagged proteins to metal affinity resins (IMAC). Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), bestatin, pepstatin A, E-64, and phosphoramidon.
Quality
One mL is recommended for the inhibition of proteases extracted from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells in a total volume of 100 ml.
This protease inhibitor cocktail has been optimized and tested for histidine-tagged proteins.
Specificity
Inhibits serine, cysteine, aspartic and thermolysin-like proteases, and aminopeptidases.
Physical Form
Solution in DMSO.