Synonym: Flavourzyme® 500L
EC Number: 232-752-2
MDL Number: MFCD00132092
Biochem/physiol Actions
Protease catabolizes proteins by hydrolysis of peptide bonds. Protease, from Aspergillus oryzae, contains both endoprotease and exopeptidase activities.
General Description
Fungal protease/peptidase complex produced by submerged fermentation of a selected strain of Aspergillus oryzae and contains both endoprotease and exopeptidase activities.
Legal Information
A product of Novozyme Corp., Flavourzyme is a registered trademark of Novozymes Corp.
Unit Definition
One unit is the amount of enzyme which hydrolyzes 1 μmol of L-Ieucine-p-nitroanilide per minute
Application
Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations. Product P6110 is a fungal protease/peptidase complex produced by submerged fermentation of a selected strain of Aspergillus oryzae. It has been injected into flies with a nanoject apparatus for infection and survival experiments.
The enzyme from Sigma has been used in the semi-purification of mouse colorectal mucins during protein digestion.