PNGase F from Elizabethkingia meningoseptica, buffered aqueous solution, recombinant, expressed in E. coli

Synonyms: PNGase F from Elizabethkingia meningoseptica; N-Glycosidase F; PNGase F from Chryseobacterium meningosepticum; PNGase F from Flavobacterium meningosepticum; Peptide N-glycosidase

CAS Number: 83534-39-8

MDL No.: MFCD00131222

Activity: activity: >=1000 U/mg

Storage: -20C

Enzyme Commission (EC) Number: 3.5.1.52  ( BRENDA | IUBMB )

UNSPSC Code: 12352204

Application: Used to deglycosylate N-linked glycoprotein.

Application: Used to deglycosylate protein.

Application: Recombinant PNGase F has been purified by affinity chromatography and dialyzed into a 50% glycerol solution with 10 mM potassium phoosphate pH 7.5 to produce a stable product. The product contains low levels of buffer salts. This highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-terminal 6x histidine fusion tag.

Biochem/physiol Actions: Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Physical form: Supplied as 300 Units/mL enzyme in 50% (v/v) glycerol and 50% (v/v) 20 mM Potassium Phosphate, pH 7.5.

Unit Definition: One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.