PluriSTEM Recombinant Vitronectin is an effective xeno-free alternative to matrigel and has been validated to successully culture human ES and iPS cells using PluriSTEM Human ES/iPS Medium (Millipore Cat. No. SCM130, SCM132), E8 and NutriStem human ES cell growth media.PluriSTEM Recombinant Vitronectin is a truncated form of human vitronectin (gene 20-398 aa fragment) that was constructed with codon optimization, expressed in E. coli and purified as inclusing bodies. The final product was refolded using a unique "temperature shift inclusion body refolding" technology and chromatographically purified. The final product is considered xeno-free.
Synonyms: S-protein, Serum-spreading factor, V75
Purity: >=95% (SDS-PAGE)
Application: Supports feeder-free and serum-free growth of human es/ips cells while maintaining xeno-free culture conditions.
Legal Information: PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany