Key to the kit is our proprietary DNA binding systems that allow the high efficient binding of DNA to our ezBind™ matrix while proteins and other contaminates are removed under certain optimal conditions. Nucleic acids are easily eluted with sterile water or Elution Buffer.
This kit is designed for fast and efficient purification of plasmid DNA from 1 to 15 mL of E. coli culture. With the binding capacity of 80 µg, the yield obtained by Miniprep Kit II (PD1213) is higher than Miniprep Kit I (PD1211). The yield from 1 mL culture is typically around 8 to 12 µg.
The purified DNA is ready for downstream applications such as cloning/subcloning, RFLP, sequencing, and transfection of HEK293 cells.