Fast and easy processing using a rapid spin-column format
No phenol or chloroform extractions
Isolate all small RNA molecules (<200 nt)
Minimal contamination from large RNA molecules and genomic DNA
High quality small RNA can be used in various downstream applications
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s Plant microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from cultured plant cells or plant tissues. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. The small RNA molecules isolated using Norgen’s Plant microRNA Purification Kit can be used in various downstream applications relating to gene regulation and functional analysis, including RT-PCR, miRNA sequencing, northern blotting and microarray analysis.