PIPES is a zwitterionic N-substituted aminosulfonic acid "Good" buffer.
PIPES is used to buffer glutaraldehyde fixative solutions when fixing plant and/or animal tissue samples. This will help prevent the loss of lipids. It is also used to buffer glutaraldehyde and formaldehyde when fixing fungal zoospores for fluorescence microscopy and electron microscopy. PIPES tends to give optimal results.
Buffers can be made by adding a solution of base to PIPES free acid, titrating to the appropriate pH.
Grade: Molecular biology reagent
Purity: ≥99%
Suitability: Suitable for cell culture
Key Applications: Buffer, Fluorescence microscopy, Electron microscopy
Application Areas: Buffer glutaraldehyde fixative solutions
Product Type: Biochemicals
Biochemical Category: Buffers
Chemical Class: Buffers
Melting Point: > 300°C(Lit.)
UV/Visible Absorbance: UV/VIS: OD260 (0.1M in 0.2M NaOH) ≤0.05; UV/VIS: OD280 (0.1M in 0.2M NaOH) ≤0.05
Presentation: White Powder
Format: Powder
pH: Effective buffering range: 6.1 - 7.5
pKa: pKa2: 6.8 at 25°C; 6.66 at 37°C(Lit.)
Detection Method: FTIR
Solubility: PIPES free acid is not very water soluble (approximately 1 g/L at 100°C) but will solubilize in 0.5 M NaOH.
Sterilization of Solutions: For sterilization it is recommended to filter sterilize using a 0.2 µm filter.
Storage & Handling: Room Temperature, desiccate