Synonyms: (4R)-Hex-5-yn-1-yl 4-((3S,5S,10R,13R,17R)-3-hydroxy-10,13-dimethyl-1,2,3,4,5,7,8,9,10,11,12,13,14,15,16,17-hexadecahydrospiro[cyclopenta[alpha]phenanthrene-6,3'-diazirin]-17-yl)pentanoate
Molecular Formula: C30H46N2O3
Molecular Weight: 482.7
Linear Structural Formula: C30H46N2O3
Purity: 95%
Storage: 2-8C
Application: This is a photo-activatable sterol probe for the bioorthogonal tagging and enrichment of sterol-binding biomolecules. This sterol probe contains an intact steroid core structure bearing a diazirine UV-activatable cross-linking group as well as an alkyne group for click-chemistry mediated attachment of reporter and affinity tags. The probes are cell-permeable allowing for in situ labeling of biomolecules in live cells as well as in cell extracts in vitro.
Other Notes: Handle the probes in amber vessels under dim ambient light whenever possible. Aqueous solutions of probes complexed to methyl-ß-cyclodextrin (20:1 m-ß-CD:probe molar ratio, 5-20 µM probe) permeate cultured cells in complete serum-containing medium within 30 minutes at 37C, and are cross-linked using near-UV (365 nm) light for 3-10 minutes. Cells can then be harvested and lysed, fractionated, or otherwise processed. Click chemistry (Cu(I)-catalyzed 1, 3-dipolar cycloaddition of the alkyne with an azide reporter tag such as a fluorophore or biotin, products #760765 or #762024) can be performed in protein samples in PBS for gel-visualization of probe-labeling, MS-based proteomics analysis of probe-mediated affinity enrichments or western blot analysis.