Polymerase chain reaction (PCR) enzymes and reagents provide a wide variety of routine, high-fidelity, and Hotstart transcription enzymes for all your PCR needs. These products support features such as reverse transcriptase cloning, cDNA from an RNA template, and high-fidelity cloning.
Featuring higher ligation specificity and lower background than Tth DNA ligase.
Eliminates LCR template-denaturation step.
The ligase is highly thermostable and features a half-life of over 60 minutes at 95°C.
Increases reliability of LCR by permitting higher melt temperatures.
Eliminates the LCR template-predenaturation step meaning that less enzyme is required for LCR.
Operates at an optimum temperature near 70°C for nick-sealing reactions
Pfu DNA ligase is a recombinant enzyme derived from pyrococcus furiosus hyperthermophilic marine archaebacterium. The recombinant DNA Ligase has a higher ligation specificity and lower background than Tth DNA ligase.
Pfu DNA ligase catalyzes linkages of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45°C to 80°C, with a temperature optimum near 70°C for nick-sealing reactions. The ligase is highly thermostable and it increases the reliability of LCR by permitting higher melting temperatures.
This product is only available to U.S. Domestic Customers.
For research use only, not to be used in diagnostic procedures.