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Jackson Immunoresearch

Peroxidase ChromPure Mouse IgG, Fab fragment

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Peroxidase ChromPure Mouse IgG, Fab fragment

ChromPure is our trade name for highly purified proteins from the serum of non-immunized animals.

Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

Host: Mouse

Product: IgG, Fab fragment

Conjugate: Horseradish Peroxidase

Physical StateFreeze-dried powder
StorageStore freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing. 
Expiration dateOne year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
PurityBased on immunoelectrophoresis at an antigen concentration of 20 mg/ml, the pattern of precipitation against goat anti-mouse whole serum is the same as that against goat anti-mouse IgG, F(ab')2 fragment specific. No precipitin line was detected against goat anti-mouse IgG, Fc fragment specific. 
Buffer0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 
Stabilizer15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) 
PreservativeNone (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.) 

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Product Detail
Thomas No.
C840F42
Mfr. No.
015-030-007
Description
Peroxidase ChromPure Mouse IgG, Fab fragment, 1 mg
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$0.00
$0.00 (0 Items)
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