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Jackson Immunoresearch

Peroxidase-AffiniPure Goat Anti-Rabbit IgG, Fc Fragment Specific

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Peroxidase-AffiniPure Goat Anti-Rabbit IgG, Fc Fragment Specific

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of rabbit IgG heavy chain but not with the Fab portion of rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
 
Physical StateFreeze-dried powder
StorageStore freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing. 
Expiration dateOne year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
PurityThe antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6  
Stabilizer15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) 
PreservativeNone (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)
Suggested Working Concentration or Dilution Range
ELISA1:5,000-1:100,000
Western Blot1:5,000-1:100,000 (non-ECL) / 1:10,000-1:200,000 (ECL)
Histo-/Cyto-Chemistry1:500-1:5,000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

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Product Detail
Thomas No.
C840M66
Mfr. No.
111-035-008
Description
Peroxidase-AffiniPure Goat Anti-Rabbit IgG, Fc Fragment Specific, 2 mL
list price/quantitytotal
$0.00
$0.00 (0 Items)
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