Anti-PD-1 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 32 kDa in Western Blots of specific cell lysates and tissues. WB validated in mouse samples, IHC and IF validated in human and mouse samples. All other applications and species not yet tested.