Anti-PARP1 (N-term ZF1) antibody with the matched secondary has been validated by western blotting and nanoimmunoassay (NIA). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 113 kDa in size corresponding to PARP-1 by western blotting in the appropriate cell lysate or extract. PARP1 (N-term ZF1) Control Protein analysis by SDS-PAGE resulted in a band at a MW ~13 kDa and estimated to be greater than 95% pure by Coomassie staining.