Papain is a sulfhydryl protease from Carica papaya latex.
- Cleaves peptide bonds of basic amino acids, leucine, or glycine
- Hydrolyzes esters and amides
- One unit will hydrolyze 1.0 µmole of N-alpha-benzoyl-L-arginine ethyl ester (BAEE) per minute at 25°C and pH 6.2
Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules.
Specific Activity: ≥10 units/mg Protein
Key Applications: For production of Fab fragments of antibodies, Used for cell dissociation
Product Type: Proteins, Enzymes & Peptides
Extinction Coefficient (E1%): 25.0 (Lit.)
Presentation: Milky White Solution
Format: Solution
Isoelectric point (pI): pH 9.6 (Lit.)
pH: Optimum pH: 6.0 - 7.0(Lit.)
Storage & Handling: Store at +4°C, do not freeze.