The Nuclear & Cytoplasmic Extraction Kit is for the enrichment of cytoplasmic and nuclear fractions from cultured cells and tissues. The kit generates a cleaner separation of cytoplasmic proteins from nuclear proteins and is ideal for expression and transport studies. This kit is based on organic buffers and contains a proprietary combination of various salts and agents. The kit is provided with reagents for solubilization of nuclear fraction. This kit is designed for300 preps, where a prep is a 20µl wet cell pellet of 2 x 10
6 cells or 200 preps of 20mg tissue.
Features
- For generation of cytosolic and ultra pure nuclear fractions.
- Provided with nuclear protein solubilization buffers.
Applications
- Extraction of cytosolic and nuclear protein fractions for transport studies, electrophoresis and other applications.