The NovaTaq™ PCR Master Mix is a ready-to-use 2X mixture of NovaTaq DNA Polymerase, ultrapure deoxynucleotides, and reaction buffer without MgCl2. The Master Mix simplifies the assembly of PCR reactions and offers advantages of time savings, consistency, and minimal risk of contamination. Simply add the NovaTaq PCR Master Mix to an equal volume containing the required amount of MgCl2, DNA template, and primers, and the reaction is ready for thermal cycling. The final diluted reaction contains 2.5 U of NovaTaq DNA Polymerase per 100 µl. Sufficient components are included for 200 standard 50-µl (or 100 × 100-µl) amplification reactions.
Components:
- 4 × 1.25 ml 2X NovaTaq PCR Master Mix
- 1.5 ml 25 mM MgCl2 Solution
- 3 × 2 ml PCR Grade Water