Nitroreductase from Escherichia coli, >=90% (SDS-PAGE), recombinant, expressed in E. coli

MDL No.: MFCD03457727

Purity: >=90% (SDS-PAGE)

Storage: -20C

UNSPSC Code: 12352204

General description: Nitroreductases can play a crucial role in redox systems via NADPH or NADH as a reductant.

Application: Nitroreductase has been used in a study that used a set of PCR primers to clone a gene encoding a hypothetical nitroreductases (named as Ssap-NtrB) from uropathogenic staphylococcus. It has also been used to improve prodrug activation. Nitroreductase from Escherichia coli has been used in a study to assess anaerobic bacteria as a gene delivery system for cancer treatment. It has also been used in a study to investigate its applications in antibody-directed enzyme prodrug therapy.

Biochem/physiol Actions: Shows ability to reduce quinines. Enzyme for activating prodrugs in antibody directed enzyme prodrug therapy.

Biochem/physiol Actions: Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical.

Physical properties: Lyophilized powder containing PBS. Does not contain BSA as excipient

Unit Definition: One unit will reduce one mumole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37 C.

Preparation Note: Produced using animal component-free materials.