Parkinson's disease is characterized by the death of dopaminergic neurons in the substantia nigra of the brain . In vitro models are critical for understanding the molecular pathology of Parkinson's disease. The N27 immortalized rat dopaminergic cell line has been widely utilized in Parkinson's research for over 20 years. However long-term passaging has led the N27 cell line to become a mixture of cell types with widely variable expression of dopaminergic neural markers such as tyrosine hydroxylase (TH).
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