Synonyms: MS Qual/Quant QC Mix
Storage: 2-8C
UNSPSC Code: 12352200
Features and Benefits: GeneralComplexity . Defined mixture gives confidence in your instruments analysisDynamic range . Generate data points that simulate the range of real world conditionsPredigested. Eliminate variabilityInjection ready. Decrease prep timeQualitativeMixture of 6 tryptically digested human proteins (FASTA file) . Low complexity, defined mixture. 25 fold concentration range. C18 purified14 SIL peptides . SIL peptides span a concentration range greater than three orders of magnitude (Table 1 ). 2 - 3 peptides per proteinQuantitative. MRM settings provided (Skyline, xls, csv). MRM transitions provided (files above). Light : Heavy ratios from 50:1 to 1:5
General description: QCAL was designed using the QconCAT technology and recombinantly expressed in E. coli.-á The parent protein sequence of QCAL is as follows:MGALRVFDEFKPLVEEPQNLIRVFDEFKPLVKPEEPQNLIRVFDEFKPLVKPEEKPQNLIRVFDEFKPLVKPEEKPQNKPLIRVFKPDEFKPLVKPEEKPQNKPLIRVFKPDEFKPLVKPEEKPQNKPLIKPRVFDEFQPLVEEPQNLIRGVNDNEEGFFSARGGVNDNEEGFFSARGGVNDNEEGFFSARGGVNDNEEGFFSARGGGVNDNEEGFFSARGGGVNDNEEGFFSARGGGVNDNEEGFFSARGGGVNDNEEGFFSARGGGVNDNEEGFFSARGVNDNEEGFFSAKGGGVNDNEEGFFSARAVMDDFAAFVEKAVMMDDFAAFVEKAVMMMDDFAAFVEKGLVKFVVPRALELFRIGDYAGIKEALDFFARYLGYLEQLLRVLYPNDNFFEGKLFTFHADICTLPDTEKALVALVLVPRGSLEVLFQGPIEGRTENLYFQGDDDDKALVALVHHHHHHQCAL was subsequently digested with trypsin to give a core mixture of 22 peptides.
Application: The MS Qual/Quant QC Mix is a LC-MS standard designed to benchmark and monitor daily performance of both qualitative and quantitative proteomic platforms. MS Qual/Quant QC Mix is an injection ready standard, designed with multiple reaction monitoring / selected reaction monitoring (MRM / SRM) methodology in mind. This product is optimized to assess platform characteristics, including:• Repeatability/Reproducibility between runs• System stability (drift, chromatography, signal intensity, sensitivity, etc.)• Inter- and intra- platform and lab comparisons
Application: MSQC2 is intended to act as a universal MS platform standard, by providing several elements for calibration and performance assessment, such as instrument resolution and linearity of signal detection.
Components: Each vial contains 25 mug of lyophilized peptides that are derived from trypsin digestion of the protein concatamer QCAL.
RIDADR: NONH for all modes of transport