KE10106 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse Total MMP-9 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Total MMP-9. The assay recognizes mouse Total MMP-9. An antibody specific for mouse Total MMP-9 has been pre-coated onto the microwells. The mouse Total MMP-9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse Total MMP-9 is added to detect the captured mouse Total MMP-9 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.