KE10069 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse sVEGFR1/Flt-1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse sVEGFR1/Flt-1. The assay recognizes mouse sVEGFR1/Flt-1. An antibody specific for mouse sVEGFR1/Flt-1 has been pre-coated onto the microwells. The mouse sVEGFR1/Flt-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse sVEGFR1/Flt-1 is added to detect the captured mouse sVEGFR1/Flt-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.