KE10064 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The KIM-1/HAVCR1 ELISA kit is to be used to detect and quantify protein levels of endogenous KIM-1/HAVCR1. The assay recognizes mouse KIM-1/HAVCR1. An antibody specific for KIM-1/HAVCR1 has been pre-coated onto the microwells. The KIM-1/HAVCR1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse KIM-1/HAVCR1 is added to detect the captured mouse KIM-1/HAVCR1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.