KE10098 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL-1 alpha ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-1 alpha. The assay recognizes mouse IL-1 alpha. An antibody specific for mouse IL-1 alpha has been pre-coated onto the microwells. The mouse IL-1 alpha protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL-1 alpha is added to detect the captured mouse IL-1 alpha protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.