KE10094 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IFN-gamma ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IFN-gamma. The assay recognizes mouse IFN-gamma. An antibody specific for mouse IFN-gamma has been pre-coated onto the microwells. The mouse IFN-gamma protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for mouse IFN-gamma is added to detect the captured mouse IFN-gamma protein. For signal development, HRP-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.