Generally, goat anti-rabbit or donkey anti-rabbit IgG polyclonal antibodies are used as secondary antibodies to detect rabbit primary antibodies. However, since polyclonal antibodies can recognize many epitopes, the background and the cross reactivity in assays is usually high, making results hard to interpret.
To minimize the background, cross reactivity, and improve the lot-to-lot consistency, a unique GenScript Mouse Anti-Rabbit IgG Fc Antibody (14H9H10)[HRP], mAb has been developed to be used as the secondary antibody. Simply substitute conventional HRP or fluorescent reagents for unparalleled clarity in results and high quality images in immunoprecipitation, Western Blot, ELISA, immunohistochemistry and immunofluorescence.