Generally, goat or donkey anti-rabbit IgG polyclonal antibodies are used as secondary antibodies to detect rabbit primary antibodies. However, since polyclonal antibodies recognize many epitopes, the background and cross reactivity in many assays is usually high, making results hard to interpret. To minimize background, cross reactivity and improve the lot-to-lot consistency, a unique Anti-Rabbit IgG Antibody (M205) has been developed to use as a secondary antibody. Simply substitute conventional HRP or fluorescent reagents for unparalleled clarity in results and high quality results in immunoprecipitation, Western Blot, ELISA, Immunohistochemistry and Immunofluorescence. Anti-Rabbit IgG Antibody (M205) only recognizes native rabbit primary IgG antibodies. This antibody does not react with denatured or reduced rabbit IgG heavy or light chain. Immunoprecipitation and Western blots often suffer from primary antibody heavy and light chain contamination at approximately 50 and 25 kDa, which is caused by poor specificity of conventional polyclonal secondary antibodies derived from goat or other species.