KE10108 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse Adiponectin ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Adiponectin. The assay recognizes mouse Adiponectin. An antibody specific for mouse Adiponectin has been pre-coated onto the microwells. The mouse Adiponectin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse Adiponectin is added to detect the captured mouse Adiponectin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.