Synonyms: Monoclonal Anti-Vinculin antibody produced in mouse
MDL No.: MFCD00162905
Storage: -20C
UNSPSC Code: 12352203
General description: Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta-subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletal muscle), neuromuscular junctions, and the membrane associated dense bodies of smooth muscle. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.
Application: Monoclonal Anti-Vinculin antibody was used in western using HS-68 total cell extract. Monoclonal Anti-Vinculin antibody was used for Immunocytochemistry using HS-68 human fibroblast cell culture.Monoclonal Anti-Vinculin antibody can be used for immunocytochemistry and Elisa.
Immunogen: purified human vinculin from uterus.
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Specificity: Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin.
RIDADR: NONH for all modes of transport