Monoclonal Anti-Mouse Tumor Necrosis Factor-a (TNF-a) is derived from the TN3-19.12 hybridoma produced by the fusion of mouse myeloma cells (P3X63-Ag8.653) and splenocytes from male Armenian hamster immunized with purified mouse recombinant TNF-a. Tumor necrosis factor (TNF) protein family contains two members: TNF-a (also known as cachectin) and TNF-ß (also known as lymphotoxin). These proteins are small molecules with a molecular weight of 17 and 25 kDa respectively and share 30% amino acid homology. TNF-a and -ß are secreted from many different cells such as lymphocytes, neutrophils, and macrophages.
Synonyms: Anti-TNF-alpha
MDL Number: MFCD01632994
Storage: -20C
Application: Monoclonal Anti-Tumor Necrosis Factor-alpha antibody produced in hamster has been used in:
•immunoblotting
•enzyme-linked immunosorbent assay (ELISA)
•immunoprecipitation
•neutralization
Biochem Physiol Actions: TNF-alpha is a major mediator of inflammatory responses and its over-production results in extensive tissue damage associated with endotoxemia and cachexia. High serum levels of TNF are associated with septic shock, rejection of renal transplants, parasitic infections, and various neoplastic diseases. TNF-alpha exhibits growth-enhancing activities for normal cells like fibroblasts, T and B cells, activation of endothelial cells, and differentiation of myeloid cells. In neoplastic cells, TNF-alpha exerts growth inhibitory activities that can be either cytostatic or cytotoxic.