Monoclonal Anti-Tyrosine Tubulin (mouse IgG3 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin as a cylindrical filamentous structure and is present in almost all eukaryotic cells. Tubulin is a heterodimer which consists of a-tubulin and ß-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology.
MDL Number: MFCD00164753
Storage: -20C
Application: Monoclonal Anti-Tyrosine Tubulin has been used in:
•indirect immunofluorescent labelling
•immunoblotting technique
•immunocytochemical staining
Biochem Physiol Actions: Tubulin acts as a major building block of microtubules. Tubulin tyrosinylation is involved in the assembly status of tubulin. A specific tubulinyl tyrosine carboxypeptidase removes the terminal tyrosine to yield an a-tubulin terminating in a glutamic acid residue while another enzyme modifies the a-tubulin by addition of tyrosine to the carboxy terminus to offer a potential cycle of tyrosine addition and loss.