Monoclonal Anti-TRF1 antibody produced in mouse, ~2 mg/mL, clone TRF-78, purified immunoglobulin, buffered aqueous solution

Synonyms: Anti-Telomeric Repeat Binding Factor 1

MDL No.: MFCD03792434

Storage: -20C

UNSPSC Code: 12352203

General description: TRF1 and TRF2 (TTAGGG Repeat binding Factors) are major proteins that bind to human telomers. TRF1 has a DNA binding domain with high homology to the Myb family of transcription factors. Unlike the Myb family that contains only one DNA binding motif, TRF1 has multiple of this motif. TRF1 has a negative effect on the length of the telomer. Over expression of TRF1 in cancer cells that contain telomerase activity, causes the shortening of the length of their telomers, while inhibition of TRF1 causes the elongation of telomers. It was shown that the level of TRF1 in the cells does not affect the expression of the telomerase protein. This suggests that TRF1 may act directly on the activity of the telomerase protein. Tankyrase is a protein that interacts with TRF1. The C-terminal region of tankyrase is homologous to PARP. In response to DNA damage, the PARP protein mediates ADP-ribose polymers of protein acceptors. In vitro studies have shown that tankyrase is responsible for that polyribosylation of TRF1, which in turn abolishes its ability to bind telomers.

Application: Monoclonal Anti-TRF1 antibody may be used in ELISA and immunoblotting (approx. 70 kDa).

Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)

Immunogen: human TRF1 protein produced in baculovirus.

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Specificity: Monoclonal Anti-TRF1 recognizes human TRF1.