Tenascin is a high molecular weight, multifunctional, extracellular matrix glycoprotein expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM).The tenascin molecule is a disulfide-linked hexamer; depending on species, the molecular weights of the subunits range from 190 to 320 kDa. In the mouse, two major subunits of tenascin with an apparent molecular weight of 210 and 260 kDa have been described. The shorter polypeptide predominates during earlier developmental stages and the larger polypeptide appears later in the embryonic gut and especially in the adult intestine. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted. It was proposed that actively growing, migrating and differentiating epithelial sheets can produce factors that can stimulate tenascin expression in the nearby mesenchyme. Human and chicken tenascin contain an RGD sequence which may function in cell adhesion and it seems likely that tenascin mediates cell attachment through an RGD dependent integrin receptor.
MDL Number: MFCD00164746
Storage: -20C
Application: Monoclonal Anti-Tenascin antibody produced in rat has been used in:
•Enzyme linked immunosorbent assay (ELISA)
•Dot blot.
•Immunoblotting
•Fluorescence microscopy and immunostaining
•Immunofluorescence
•Immunohistochemistry
Biochem Physiol Actions: Tenascin is a high molecular weight, multifunctional, extracellular matrix glycoprotein expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted. It was proposed that actively growing, migrating and differentiating epithelial sheets can produce factors that can stimulate tenascin expression in the nearby mesenchyme. Human and chicken tenascin contain an RGD sequence motif which may function in cell adhesion and may be recognized by integrin receptor.