Synonyms: Monoclonal 6 His epitope tag; Monoclonal 6xHis-tag; Monoclonal HHHHHH epitope tag; Monoclonal Hexa His tag; Monoclonal His-tag; Monoclonal His6 tag; Monoclonal Histidine tagged; Monoclonal Poly-His-tag
Storage: -20C
UNSPSC Code: 12352203
General description: Monoclonal Anti-polyHistidine (mouse IgG2a isotype) is derived from the HIS-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant His-tagged fusion protein. The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor. Monoclonal anti-polyHistidine recognizes native as well as denatured, reduced forms of synthetic polyHistidine or polyhistidine-tagged fusion proteins. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET, and pTrc expression vectors. The antibody preferentially recognizes N-terminal tagged fusion proteins.
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.
Other Notes: In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration. Use of sensitive film is recommended.
RIDADR: NONH for all modes of transport
WGK Germany: 1