Monoclonal Anti-phospho-RNA polymerase II CTD (pSer5) (rat IgG2b isotype) is derived from the hybridoma 1H4B6 produced by the fusion of mouse myeloma cells and lymph node cells from rat immunized with Synthetic peptide.. The C-terminal domain (CTD) RNAPII also known as POLR2A is the largest subunit of RNA polymerase II. It comprises the heptapeptide repeats with serine and threonine residues in the C-terminal. POLR2A gene is mapped to human chromosome 17p13.1.
Synonyms: CCFDN; CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase; CTD of POLR2A; FCP1; POLR2; POLRA; RNA polymerase II subunit A C-terminal domain phosphatase; RPB1; RPBh1; RPO2; RPOL2; RpIILS; TFIIF-associating CTD phosphatase 1; hRPB221; hsRPB1; phosphatase of, subunit 1; polymerase (RNA) II (DNA directed) polypeptide A 220kDa (POLR2A); serine phosphatase FCP1a; subunit 1CTDP1; transcription factor IIF-associating CTD phosphatase 1
Storage: -20C
Application: Monoclonal Anti-phospho-RNA polymerase II CTD (pSer5) antibody produced in rat may be used in immunoblotting and immunofluorescence.
Biochem Physiol Actions: The C-terminal domain (CTD) of the RNA polymerase II is essential for polymerase activity and gene regulation.. The CTD functions to help couple transcription and processing of the nascent RNA and also plays roles in transcription elongation and termination. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. CTD is being dynamically modified during transcription cycle and the Ser 5 phosphorylation in promoter region is more prominent than the 3' region. The phosphorylation at Ser5 and Ser7 is mediated by the cyclin dependent kinase 7 of the transcription factor II human (TFIIH). The recruitment of the capping machinery is the main function of phosphor-Ser5. However, other protein interactions require the phosphor-Ser5 as well. Mutation in the domain regions of POLR2A is implicated in developmental delay and in neurodevelopmental syndrome.