Synonyms: Anti-CDKN1A; Anti-Cip1; Anti-WAF1; Anti-p21
MDL No.: MFCD00802813
Storage: -20C
UNSPSC Code: 12352203
General description: Mouse monoclonal clone CP74 anti-p21WAF1/Cip1 antibody reacts specifically with p21WAF1/Cip1. It shows no cross-reactivity with other closely related mitotic inhibitors. The epitope recognized by the antibody resides in the N-terminal amino acids 17-30 within the cyclin-kinase binding domain of the p21WAF1/Cip1 molecule. Reactivity has been observed with human and mouse p21WAF1/Cip1.
General description: Regulation of cell cycle progression in eukaryotic cells depends on the expression of proteins called cyclins. These proteins form complexes with several different cyclin dependent kinases (CDKs). The catalytic activity of cyclin dependent kinases (CDK) is regulated by two general mechanisms: protein phosphorylation and association with regulatory subunits, which include the cyclins and the CDK inhibitors (CKIs). Two families of CKIs have been identified. The p21WAF1/Cip1 family contains p21WAF1/Cip1, p27Kip1 and p57Kip2. These inhibit all kinases involved in the G1/S transition. The the p16INK4a family, which includes p15INK4b, p16INK4a, p18INK4c and p19INK4d, inhibits Cdk4 and Cdk6 specifically.
Application: Mouse monoclonal clone CP74 anti-p21WAF1/Cip1 antibody may be used for ELISA, immunoblotting (21 kDa) and immunoprecipitation (p21/cyclin A/Cdk2 complexes, native and denatured). The availability of a monoclonal antibody reacting specifically with p21WAF1/Cip1 enables the subcellular detection and localization of p21WAF1/Cip1 and the measurement of relative differences in p21WAF1/Cip1 levels as a function of cell cycle phase.
Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Immunofluorescence (1 paper)Western Blotting (1 paper)
Immunogen: recombinant human p21WAF1/Cip1.
Physical form: Solution in phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide
RIDADR: NONH for all modes of transport
WGK Germany: 3