Monoclonal anti-green fluorescent protein (GFP) (mouse IgG1 isotype) is derived from the GFP-20 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse BALB/c mice immunized with a GFP tagged fusion protein.GFP is a 27kDa protein, derived from the bioluminescent jellyfish Aequorea victoria, in which light is produced when energy is transferred from the Ca2+-activated photoprotein aequorin to GFP.
Synonyms: green fluorescent protein
Storage: -20C
Application: Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse may be used in:
• immunoblotting
• immunoprecipitation
• dot blot
• enzyme-linked immunosorbent assay (ELISA)
• scanning electron microscope (SEM)
Biochem Physiol Actions: GFP (green fluorescence protein) is a reporter molecule which is used for checking gene expression and protein localization. GFP emits green light when it is excited with UV/blue light. The GFP fluorescence remains stable and can be detected non-invasively in living cells. GFP is considered as a unique tool to monitor dynamic processes in several living cells or organisms. When expressed in either eukaryotic or prokaryotic cells and illuminated by blue or UV light, GFP yields a bright green fluorescence.