Monoclonal Anti-Gelsolin antibody produced in mouse, clone GS-2C4, ascites fluid

MDL No.: MFCD00164595

Storage: -20C

UNSPSC Code: 12352203

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Application: Monoclonal Anti-Gelsolin antibody produced in mouse is suitable as a primary antibody in western blotting . for the detection of gelsolin lysates and supernatants of UVB-irradiated keratinocytes to examine whether caspase-4 expression is required for UVB-induced unconventional protein secretion. to measure plasma gelsolin levelsIt is suitable for immunoblotting at a working dilution of 1:1000 using human plasma extract. It may be used for ELISA and immunofluorescent staining of frozen sections for detection of gelsolin-actin complexes. This antibody has also been used in immunoaffinity chromatography for purification of gelsolin and for detection of gelsolin-actin complexes in cell extracts.

Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)

Biochem/physiol Actions: Gelsolin is a calcium dependent actin binding protein. It is a potent modulator of actin filament length and gelation. It binds to the ′plus′ ends of actin monomers and filaments, preventing monomer exchange. It exists in two variant forms, cytoplasmic gelsolin and plasma gelsolin. Plasma gelsolin is also called actin depolymerizing factor (ADF) or brevin. Mutations in this gene cause familial amyloidosis Finnish type (FAF) characterized by cranial neuropathy and lattice corneal dystrophy. Gelsolin is used as a biochemical marker for acute rheumatic carditis.

Immunogen: human plasma gelsolin.

Specificity: The antibody is specific for an epitope located on the 47 kDa peptide derived from a chymotryptic cleavage of gelsolin. The antibody reacts with plasma and cytoplasmic gelsolin. An additional application for this antibody is immunoaffinity chromatography, for purification of gelsolin, and for detection of gelsolin-actin complexes in cell extracts.

Target description: Gelsolin is an actin-severing protein found in mammalian cells and blood plasma. Has tumor suppressor activity in several cancer cell lines and in nude mice.