Monoclonal Anti-Human Fibronectin (mouse IgG1 iso-type) is derived from the IST-3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c immunized mice. Fibronectin (FN) is a multifunctional extracellular matrix protein comprising two nearly-identical disulfide-bound polypeptides. It is an adhesive glycoprotein. FN belongs to the ligand glycoprotein family and is is expressed in various cell types. Human fibronectin consists of five distinct domains that are referred to as heparin (Hep)-1/ fibrin (Fib)-1, gelatin (Gel), cell surface (Cell), Hep-2 and Fib-2, based on their affinity for Hep, Gel, Cell or Fib. The FN gene is mapped to human chromosome location 2q35.
MDL Number: MFCD00162291
Storage: -20C
Application: Mouse monoclonal clone IST-3 anti-Human Fibronectin antibody has been used in:
•enzyme linked immuno sorbent assay (ELISA)
•western blotting
•dot blot
•radio immunoassay (RIA)
•immunocytochemistry
•immunohistochemistry
•immunoprecipitation
•immunofluorescence
Biochem Physiol Actions: Fibronectin (FN) binds to cell surfaces and a variety of molecules, such as collagen, heparin, gelatin, fibrin, and DNA. It plays an important role in diverse biological phenomena including cell adhesion, cell migration, hemostasis, and thrombosis. FN is also involved in wound healing and stimulates a normal phenotype in transformed cells. Upregulation of FN leads to tumors, such as nasopharyngeal carcinoma (NPC), ovarian cancer, and head and neck squamous cell carcinoma.