Monoclonal Anti-Pan Cytokeratin (clone C-11) recognizes human cytokeratins 4, 5, 6, 8, 10, 13 and 18 in immunoblotting. The antibody reacts with simple, cornifying and non-cornifying squamous epithelia and pseudostratified epithelia. It does not react with non-epithelial normal human tissues. This antibody can be applied to methanol or acetone-fixed, frozen sections, and to formalin-fixed, paraffin-embedded human tissues. Increased staining intensity is seen following proteolytic treatment of formalin fixed tissue. Similarly, methacarn-fixed material is also suitable for cytokeratin demonstration. Monoclonal Anti-Pan cytokeratin exhibits a wide interspecies cross-reactivity (e.g., human, bovine, rat, frog).
Synonyms: Monoclonal Anti-Pan Cytokeratin
Storage: -20C
Application: Monoclonal anti-cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Mouse monoclonal clone C-11 anti-cytokeratin, pan antibody is a broad spectrum antibody which recognizes an epitope present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. It may aid in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues, and for determining the origin of poorly differentiated tumors. It is useful for staining of cultured epithelial cell lines. Mouse monoclonal clone C-11 anti-Cytokeratin, pan antibody may be used for the localization of cytokeratins using various immunochemical assays such as immunoblotting, dot blotting and immunohistochemistry (immunofluorescence and immunoenzymatic staining).
Biochem Physiol Actions: Cytokeratins are usually used to determine epithelial differentiation. They help to maintain the integrity of the epithelium of the anterior segment of the eye.