Monoclonal Anti-CYBA (mouse IgM isotype) is derived from the hybridoma CB-64 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the internal region of human CYBA, conjugated to keyhole limpet hemocyanin (KLH). The small subunit, p22phox subunit, also known as cytochrome b245, alpha polypeptide/light chain (CYBA) is membrane bound,which is expressed in phagocytic, endothelial and vascular smooth muscle cells. This gene is located on human chromosome16q24
Synonyms: Cytochrome b558 subunit alpha; Neutrophil cytochrome b 22 kDa polypeptide; Superoxide-generating NADPH oxidase light chain subunit; cytochrome b-245 alpha polypeptide; p22-PHOX
Storage: -20C
Application: Monoclonal Anti-CYBA antibody produced in mouse has been used in:
•immunoblotting
•flow cytometry
•immunofluorescence
Biochem Physiol Actions: In prostate cancer cells downregulation of cytochrome b245, alpha polypeptide/light chain (CYBA) was reported to inhibited cell proliferation and colony formation, through AKT and ERK1/2 signaling pathways, indicating CYBA has a role in tumor angiogenesis and tumor growth. Mutations in this gene causes an autosomal recessive forms of chronic granulomatous disease (AR220CGD).