Monoclonal Anti-b-Tubulin (mouse IgG1 isotype) is derived from the TUB2.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with purified rat brain tubulin. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Tubulin is a heterodimer, which consists of a-tubulin and ß-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. ß-tubulin structure is characterized with the core of two ß-sheets enclosed by a-helices.
Synonyms: Monoclonal Anti-beta-Tubulin
Storage: 2-8C
Application: Monoclonal Anti-ß-Tubulin-Cy3 antibody produced in mouse has been used in:
•immunohistochemical assays
•immunofluorescence
•western blotting
•immunocytochemistry
•immunoblotting
•immunostaining
Biochem Physiol Actions: Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Mutation in the ß-tubulin gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations.
Legal Information: Cy is distributed under license from Amersham Biosciences Limited.