Monoclonal Anti-ß-Galactosidase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. ß -galactosidase is a E coli protein and is a tetramer with 1023 amino acids.
Synonyms: Monoclonal Anti-beta-Galactosidase
Storage: -20C
Application: Monoclonal Anti-ß-Galactosidase antibody produced in mouse has been used in:
•immunostaining and histochemistry
•immunostaining and microscopy
•immunohistochemistry
•immunofluorescence staining
•enzyme linked immunosorbent assay(ELISA)
•immuno dot blot
Biochem Physiol Actions: ß -galactosidase substrate is lactose. Synthetic substrates like o-nitrophenyl-ß-d-galactopyranoside (oNPG) or p-nitrophenyl-ß-d-galactopyranoside (pNPG) are used in enzymatic assays. It is also used as marker in studying gene expression. The use of monoclonal ß -galactosidase anti- ß -galactosidase (BGABG) soluble complex in the unlabeled antibody-enzyme method results in an intense signal with a very low background.