Monoclonal Anti-beta-Galactosidase antibody produced in mouse, clone GAL-40, ascites fluid

MDL No.: MFCD00162155

Storage: -20C

UNSPSC Code: 12352203

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General description: β-galactosidase is a ~465 kDa tetrameric protein. Eukaryotic genes are usually cloned into E.coli β-galactosidase (lacZ) gene that results in a fusion hybrid of the desired gene product with the β-galactosidase enzyme . As the amount of the desired gene product in these cloned vector systems corresponds to the amount of β-galactosidase, enzyme assays for β-galactosidase can be utilized to detect the required gene product . Monoclonal anti-β-galactosidase antibodies are specific for β-galactosidase in E.coli and enable the analysis of β-galactosidase fusion proteins encoded by cloned vector systems.

General description: β-Galactosidase is coded by the LacZ gene which helps to transform lactose into galactose.

Application: Monoclonal anti-beta-galactosidase antibodies may be used in dot blot (using soluble beta-galactosidase), western blot (at a dilution of 1:1,000, using denatured-reduced Escherichia coli beta-galactosidase) and immunocytochemistry. This antibody may also be used for immunochemical assays such as ELISA, immunoblot and immunohistochemistry by using the stepwise procedure or by the preparation of beta-galactosidase anti-beta galactosidase (BBAG) complex. Additionally, this product is suitable for the detection and purification of recombinant fusion proteins encoded by cloning vectors containing the beta-galactosidase gene. Monoclonal anti-beta-galactosidase antibody is also used for the immunoenzymatic staining of bone marrow and blood smears, and tissue sections.

Immunogen: beta-D-galactosidase from E. coli

Specificity: The antibody may be used for detection of beta-galactosidase expressed by the E. coli lacZ gene encoded in many cloned gene sequences; serves as an indicator for fusion proteins encoded by an inserted DNA sequence.