Monoclonal Anti-ARP3 (mouse IgG2b isotype) is derived from the hybridoma FMS338 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with recombinant human ARP3. Arp3 is mapped to human chromosome 2q14.1.
MDL Number: MFCD04118400
Storage: -20C
Application: Monoclonal Anti-ARP3 antibody produced in mouse has been used in:
•enzyme linked immunosorbent assay (ELISA)
•immunocytochemistry
•immunoblotting
•immunoprecipitation
•immunofluorescence
•immunohistochemistry
Biochem Physiol Actions: The actin-related protein-2/3 (ARP2/3) is part of Wiskott-Aldrich Syndrome protein (WASP) signaling in mediating polymerization state of actin filaments. It has been found that ARP2/3 complex and its activator spinocerebellar ataxia, autosomal recessive 2 (Scar2) are involved in Golgi polarization in NIH 3T3 cells. However in primary astrocytes, Golgi polarization involves neither actin cytoskeleton or Arp2/3 complex nor any WASP-family of proteins. In a different model, it was found that the ARP2/3 complex was necessary for neutrophil chemotaxis and phagocytosis. By genetic and loss of function studies, it was demonstrated that Scar2 is the major regulator of the ARP2/3 complex rather than the WASP family of proteins, the latter having more restricted roles in specific cellular events.