alpha-actinin is a 100kD actin binding protein found in muscle as well as non-muscle cells. In smooth muscle, alpha-actinin is identified in dense bodies and plaques characteristic of tissues whereas it is associated with z-discs that define muscle sarcomas in normal skeletal muscle. Monoclonal anti-alpha-actinin antibody can be used in immunoblotting to study the membrane anchorage sites and immunochemical identification of alpha-actinin. It can also be used in immunofluorescence to study the localization of alpha-actinin in cultured cells and tissues. Mouse anti-alpha-actinin antibody reacts specifically with chicken fibroblasts. The product has also shown reactivity for mouse and human cells.
MDL Number: MFCD00162107
Storage: -20C
Application: Monoclonal anti-a-actinin antibody (diluted 1: 500) can be used in native protein binding assays. It can also be used in western blot, microarray, immunohistochemistry and in Immunofluorescence microscopy.
Biochem Physiol Actions: a-actinin regulates muscle contraction and cytoskeletal organization. It promotes cell migration and acts as an actin crosslinker. Mutations in the a-actinin gene is observed in heterogeneous hypertrophic cardiomyopathy and in juvenile onset atrial fibrillation.